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VECTOR
INSERT
pET21b
MTD
~20 amino acid
Name : Clostridium botulinum neurotoxin type A light chain
Lc
Amino acid: 447 amino acid
Sequence : Clostridium botulinum type A Hall Lc
Tag
Hexa-histidine
우리의 원료
'Methionyl r-Clostridium
Botulinum Polypeptide-1 Hexapeptide-40' 의 구조
목표세포 침투 기능을 가진 'MTD" (Macro-molecule Transmission Domain) 펩타이드와 '보툴리눔 톡신의 활성 단백질인 'Light Chain'의 재조합으로 개발되었습니다. 우리 원료에 적용된 'MTD'는 보툴리눔 톡신의 세포투과 기능을 담당하는 'Heavy Chain'의 기능에서 비롯되어 개발되었습니다.
STRUCTURE
MTD
Lc
His
469aa(53.6kDa)
보툴리눔 톡신 원료의 비교
Toxin Complex
(Medical Botox Injection)
Botulinum Light Chain A
Botulinum Heavy Chain
Hemaggultinin
Non-toxin
Non-toxin Non-Hemagglutinin
Composition
Heavy chain : Nerve cell penetration
Light chain : Botulinum Efficacy Part
Hemagglutinin
Non-toxin Non-Hemagglutinin
Commercialization
1980
Example
BOTOX (Allergan, US)
MEDITOXIN (Meditox, Korea)
Not permitted to use in Cosmetic Product
Molecular weight
900KDa
Pure Toxin
(Medical Botox Injection)
Botulinum Light Chain A
Botulinum Heavy Chain
Composition
Heavy chain : Nerve cell penetration
Light chain : Botulinum Efficacy Part
Commercialization
1995
Example
XEOMIN (Merz, Germany)
Not permitted to use in Cosmetic Product
Molecular weight
150KDa
Our Ingredient
(BPMed_BoLCA_Protein)
Botulinum Light Chain A
MTD Protein
Composition
Light Chain + MTD
Botulinum Efficacy Part
Marco-molecule Transmission Domain
(Skin & Nerve cell penetration)
= Recombination of the Protein
Commercialization
2014
Example
BoLCA+ (BPMed cosmetic, Korea)
The First Botulinum Ingredient on ICID
BPMed Cosmetic has Exclusivity
Molecular weight
50KDa
MTD의 피부 및 세포투과 기술
'Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40' 의 경피 전달 향상을 위한 플랫폼 테크놀러지
1st Action
MTD에 의한 각질층 및 다른 피부층으로의 'Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40' 침투향상
2nd Action
특정 목표지점으로 'Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40' 을
전달함으로써 효능 극대화
Epidermis
Dermis
Subcutaneous Tissue
Muscle Layer
Cell
신경세포에 대한 작용 메커니즘
Normal Neurotransmission Process
SNARE Complex Exocitosis Acetylcholine Diffusion
Binds to the Receptors on the Muscle
Nerve Cell
Approaching to the Cell
SNAP 25
Moves to the SNAP25
Penetrated in to dermis layer
Methionyl r-Clostridium Botulinum
Polypeptide-1 Hexapeptide-40
Direct Cell Membrane Translocation
Cleaves SNAP25 by Light Chain Part